Enrichment of peripheral blood mononuclear cells (PBMC) Read more
Authors: Ulrika Andreasson
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Protein purification is a crucial step for projects involving research on structural and functional properties. The quality of the protein cannot be compromised. We offer a comprehensive service of purification of proteins up to the highest level of purity required. Read more
Authors: Lily Green
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http://www.creative-proteomics.com/ProteinIdentification.htm Read more
Authors: Lily Green
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De Novo Peptide Sequencing is a tech to analyze proteins or peptides not present in currently available databases. We use tandem mass spectrometry (MALDI TOF-TOF, or electrospray MS/MS) to determine the primary sequence structure. The expert operators will then use the PEAKS Software to interpret the MS/MS spectra and derive the customer sequence. Read more
Authors: Lily Green
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PTMs(hereafter): Phosphorylation (pS/T, pY), Methylation, Deamidation, Oxidation, Nitration, N-glycosylation, Amino acid mutation, Unnatural amino acid, Chemical modifications, Palmitoylation, Glycosylation, Ubiquitination, SUMOylation, Dimethylation, Acetylation, Decarboxylation, etc.. Read more
Authors: Lily Green
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This service is a quantitative and qualitative analysis of two or more complex samples such as cell lysates, tissue homogenates or biofluids. The results from this experiment provide a catalogue of the proteins present in all samples and a statistical analysis reflecting the changes in the protein levels observed across the samples, along with associated pathway information. Read more
Authors: Lily Green
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Discovery and validation of protein and peptide biomarkers in clinical proteomics requires large numbers of patient samples. Traditional methods are complicated, time-consuming and lack standardization. Peptide profiling with magnetic chromatography beads offers quick, reproducible screening and can be automated to run 96 samples in a day from start to finish. The method is reproducible and can be standardized, facilitating clinical and inter-laboratory collaborations. Read more
Authors: Lily Green
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Discovery and validation of protein and peptide biomarkers in clinical proteomics requires large numbers of patient samples. Traditional methods are complicated, time-consuming and lack standardization. Peptide profiling with magnetic chromatography beads offers quick, reproducible screening and can be automated to run 96 samples in a day from start to finish. The method is reproducible and can be standardized, facilitating clinical and inter-laboratory collaborations. Read more
Authors: Lily Green
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N-terminal Sequencing (also called Edman sequencing) uses a chemical process based on the technique developed by Pehr Edman in the 1950's. N-terminal sequencing is a method to excise amino acid one by one from N-terminal of protein or peptide to be segregated by HPLC (reversed phase HPLC), then to detect by UV to determine amino acid sequence by the time the peak is gained (Chromatogram). Pure proteins (>90%) usually generate easily interpreted data, but insufficiently purified protein mixtures may also provide useful data. Long sequences of 50 amino acids or more are possible with this technique. Read more
Authors: Lily Green
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This SOP addresses the titration of VSV or Maraba virus by plaque assay on VERO cells. Read more
Authors: Charles Lefebvre
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