This protocol is designed to perform high-throughput metabolic profiling analysis on human blood plasma by ultra-performance liquid chromatography/mass spectrometry (UPLC/MS). In the development of the protocol, UPLC columns with three different stationary phases (C8, C18, and phenyl) were used in identical experimental runs consisting of a total of 60 injections of extracted male and female plasm...

Authors: Stephen J Bruce et al
+1
+1
-1

This protocol describes the procedure for a direct labeling of proteins in complex plasma, serum or urine samples. Samples can be either labeled directly by a fluorescent dye, or by a hapten such as biotin using NHS-ester chemistry.

Authors: Cristoph Schröder Schröder et al
+1
0
-1

This is an efficient and basic protocol for Western Blotting using the iBlot® 7-Minute Blotting System and ChemiDoc-IT imaging system to perform Western Blotting.

Authors: Francisco Mansilla
+1
0
-1

A protocol for extraction of blood plasma/serum to create dry samples.

Authors: Linus Malm
+1
0
-1

This protocol is designed to perform high-throughput metabolic profiling analysis on human blood plasma by ultra-performance liquid chromatography/mass spectrometry (UPLC/MS). In the development of the protocol, UPLC columns with three different stationary phases (C8, C18, and phenyl) were used in identical experimental runs consisting of a total of 60 injections of extracted male and female plasm...

Authors: Stephen J Bruce et al
+1
0
-1

The following protocol is intended for the simultaneous extraction of DNA and RNA from various soil samples along with suggestions on how to tweak the protocol for soil with higher humic content. The protocol has been used by many and results in very high yields of nucleic acids, typically much more than commercial kits. For buffers and solutions used in this protocol, please also check the accomp...

Authors: Roey Angel
+1
0
-1

Optogenetics has evolved to be a critical technique used to manipulate the firing activity of specific subsets of neurons. Through the use of specific firing parameters, it has become possible to control the behavior of freely moving animals. Here we have established a system to control projection pathway-specific neurons from a particular brain region through the combined use of a Cre-dependent ...

Authors: Jessica Walsh Dispesh Chaudhury et al
+1
0
-1

Retrograde dyes, such as lumafluors, have been used as tracers to visualize neurons that project to a specific target region. Injection of these dyes provides an important method in being able to understand the functional role of projection-specific neurons. Lumafluors can be directly injected into a target brain region of a mouse and dye positive cells from the projection region can be electrophy...

Authors: Jessica Walsh et al
+1
0
-1

We report a simple gram scale synthesis of several imidazolinium salts (Br, Cl, PF6 and BF4), the synthesis of N,N’–bis–(2,4,6–trimethylphenylamino)ethane dihydrobromide is described in this protocol

Authors: Arnaud Gautier et al
+1
0
-1

We report a simple gram scale synthesis of several imidazolinium salts (Br, Cl, PF6 and BF4). This particular protocol describes the synthesis of N,N’–bis–(2,4,6–trimethylphenylamino)ethane dihydrochloride

Authors: Arnaud Gautier et al
+1
0
-1

Pages