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Home » Protocols » RNase free PBS

Protocol

RNase free PBS

Acc.no: M71QS1 | Published: 2008-12-01 by Unknown user

Keywords: PBS, RNase, RNase-free

Describes the preparation of RNase free PBS.

Whether the PBS is RNase-free or not depends on the grade of water used and the protocol can of course be adapted to suite ones needs.

Required Materials

Main steps

  1. Procedure

Instructions

  1. Procedure
    8.0 gNaCl
    0.2 gKCl
    1.44 gNa2PO4
    0.24 gKH2PO4
    ? 1 literDEPC-H2O

    Dissolve NaCl, KCl, Na2PO4 and KH2PO4 in 800 ml of DEPC-H2O. Adjust the pH to 7.4 using HCl.

    To avoid contamination by RNase this is preferably done by dropping the solution on a pH strip.

    Adjust the volume to 1 liter with DEPC-H2O. Sterilize by autoclaving.

Contacts

Protocol has no contact information.

Attachments

None.

History

Created by jennie on 2008-12-01.

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References

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