Home » Protocols » Tris-HCl buffer
Protocol
Tris-HCl buffer
Acc.no:
LY9PV5
|
Published:
2008-11-28
by
Unknown user
Keywords: tris, tris-buffer, Tris-HCl, buffer
Tris-HCl buffer is commonly used for biochemial experiments.
Instructions
-
General information
Name Tris base, Tromethamine Molecular Weight 121.13504 g/mol Molecular Formula C4H11NO3 XLogP -2.7 CAS No. 77-86-1 pKa(25ºC) 8.08 -
Preparation
- Prepare 800 mL of base solution.
- Add HCl soln. according to target pH.
- Adjust the pH of the solution.
- Add H2O up to 1L.
-
Acid
target pH volume amount 7.4 70 mL 12×0.07 = 0.84 mol 7.6 60 mL 12×0.06 = 0.72 mol 8.0 42 mL 12×0.042 = 0.50 mol HCl soln: 12M, 37% w/w, hazardous!
