Svanodip ® FMDV-Ag penside test

Acc.no: L7HM13 | Published: 2008-11-19 by Unknown user | Author: Ann Nordengrahn

Keywords: Foot and Mouth Disease Virus, Foot and Mouth Disease, FMVD, Antigen, Penside

Penside test for the detection of FMVD (Foot and Mouth Disease Virus) antigen in swab and tissue samples

The FMDV (Foot and Mouth Disease Virus)antigen test is a simple direct test for the detection of all seven serotypes of the FMDV antigen in clinical samples and can be carried out at the location of the farm.

In this assay FMDV antigen specific antibodies have been bound to colloid gold as well as immobilised on the membrane. If present in the sample, FMDV antigen binds to the gold conjugate forming an immune complex. The complex then migrates by capillary action along the membrane until it reaches the immobilised antibody. The complex will bind to the immobilised antibody, resulting in an accumulation of colloid gold (a red/purple line) visible by eye. A band in the Test (T) window indicates a positive result. No band in the Test (T) window indicates a negative result. The antibody in the Control (C) window always binds colloid gold-antibody, regardless of FMDV viral presence. This C-band ensures correct test performance.

Required Materials

Instructions

Specimen collection and processing

Vesicular fluid A small volume of vesicular fluid is collected by a syringe and the amount is calculated. An equal amount of sample dilution buffer is added to the sample and mixed.

Epithelium Approximately 0.2 g of epithelium (the size of the nail on a little finger) is collected from the surface or margins of vesicles. Best results will be achieved with fresh and friable material. Add 2 ml of sample buffer into the glass bottle. Place the epithelium into the glass bottle and vigorously shake four times over a five minute period, with the aim of liquidizing the material.
Precautions
1. Carefully read and follow all instructions.
2. Store the kit and all reagents at +4 to +28°C (39 to 82°F)
3. All reagents should equilibrate to room temperature 18 to 25°C (64 to 77°F) before use.
4. Handle all materials as though capable of transmitting infectious disease.
5. Do not mix components or instruction booklets from different test kits.
6. Care should be taken to prevent contamination of kit components.
7. Do not use test kit beyond date of expire.
8. Do not eat, drink, or smoke where specimens or kit reagents are handled.
9. Use a separate pipette tip for each sample.
10. Burn all unused biological materials before disposal.
Procedure
1. Make sure that the device is at ambient temperature before opening the bag.
2. Remove the test device from the bag and place on a flat surface.
3. Use the pipette to take prepared sample solution (see point 3.1) up to the mark on the pipette. Hold vertically and slowly add five drops (approximately 200µl) of the sample solution to position ”S” on the test device.
4. Read results within 5 - 10 minutes. Disregard any lines which appear after 45 minutes.
Interpretation of the results
1. A line in the Control (C) window shows that the test has worked correctly. Any device that has not produced a control line at 10 minutes must be classified as void.
2. A line in the Test (T) window indicates a positive result and that the sample contains FMDV antigen. If no line appears in the Test (T) window the test is negative for FMDV antigen (see Figure below).
3. A difference in the intensity may occur between the line in the Test (T) and Control (C) window but this does not affect the interpretation of the result.
Figure penside test
Full size