Collection of Blood Serum

Acc.no: 19J5LGB | Published: 2009-09-03 by jennie | Affiliation: IARC - International Agency for Research on Cancer

Keywords: whole blood, collection, blood

The protocol is derived from the WHO/IARC guideline “Common Minimal Technical Standards and Protocols”. The purpose of the document is to provide a framework for the development and coordination of Biological Resource Centers.

The protocol describes the collection of blood without added anticoagulants.

The blood is collected into tubes without addition of anticoagulants. Then two phases are originating, a solid phase, containing fibrin and cells, and a fluid phase, containing the serum.

This process should be completed after 30 minutes at room temperature, and then the process described below starts.

Required Materials

Centrifuge
sterile 15ml centrifuge tubes
Cryovial
Liquid Nitrogen
Freezer (-80) , or liquid nitrogen storage
Liquid Nitrogen , or dry ice

Instructions

Centrifuge blood

Spin blood in 15ml sterile tubes at 1500xg for 10 minutes at room temperature.
Aliquot into cryovials

Aliquot 1ml portions of supernatant into labeled cryovials.
Snap freeze

Place ryovials into liquid nitrogen Dewar or dry ice to snap freeze.
Store cold

Transfer to -80°C freezer or liquid nitrogen storage.