The discussions are divided into two cathegories, protocol specific and general .

Title	Comments	Modified	Started
How can we make MolMeth better?	1	2009-04-02	2009-04-02
Cell numbers in permanox chamber slides	1	2011-06-25	2011-06-25

Protocol specific discussion

These discussions are also accessible from their respective protocol.

Acc.no.	Title	Comments	Modified	Started
5Y0Q64	PSCP GA	0	2008-12-12	2008-12-12
5Y4W2B	Proximity ligation assay	0	2008-12-12	2008-12-12
5Y8JWT	Dual tag microarray	0	2008-12-12	2008-12-12
5YC5JK	In situ genotyping of mitochondrial DNA using padlock probes and target-primed RCA	0	2008-12-12	2008-12-12
5YGGQC	Padlock probe ligation and preparation for dual tag microarray readout	0	2008-12-12	2008-12-12
MF43XA	Urine samples for mosaiques CE-MS analysis	0	2008-12-12	2008-12-12
M71QS1	RNase free PBS	0	2008-12-12	2008-12-12
LY9PV5	Tris-HCl buffer	0	2008-12-12	2008-12-12
L7M8DP	Type A, H5, and H7 Avian Influenza antigen detection ELISAs	0	2008-12-12	2008-12-12
L7LEGC	Mab based competitive ELISAs for H5, and H7 antibody detection in avian sera	0	2008-12-12	2008-12-12
L7K2C3	FMDV PLA assay	0	2008-12-12	2008-12-12
MF4C33	Isolating Genomic DNA from Whole Blood	0	2008-12-12	2008-12-12
MF548L	DNA Ligation Using T4 DNA Ligase and Bacterial Transformation	0	2008-12-12	2008-12-12
MF60C2	Two-Step RT-PCR Protocol	0	2008-12-12	2008-12-12
MF6M41	One-Step RT-PCR Protocol	0	2008-12-12	2008-12-12
MF6V09	Basic PCR protocol	0	2008-12-12	2008-12-12
MVA3YJ	ELISA buffers	0	2008-12-12	2008-12-12
MXPE74	Coating buffer (ELISA)	0	2008-12-12	2008-12-12
MXY70G	Chromophore/Substrate solution (ELISA)	0	2008-12-12	2008-12-12
L7HM13	Svanodip ® FMDV-Ag penside test	0	2008-12-12	2008-12-12
L7H5PV	CSFV Specific Real Time RT-PCR	0	2008-12-12	2008-12-12
L7G20D	Bluetongue SYBR-Green RT-PCR	0	2008-12-12	2008-12-12
5YGY1L	Solid phase proximity ligation assay (S-PLA)	0	2008-12-12	2008-12-12
8K7NAR	PCR Product Cloning Using T Vectors	0	2008-12-12	2008-12-12
L1MTXC	Swine vesicular disease virus one-step RT-LAMP	0	2008-12-12	2008-12-12
L1TT4C	One-step RT-LAMP assay	0	2008-12-12	2008-12-12
L2AYP3	Swine vesicular disease virus PriProET two-step real-time PCR	0	2008-12-12	2008-12-12
L2B9FW	PriProET two-step real-time PCR	0	2008-12-12	2008-12-12
L2BHWT	Ribonucleic acid extraction from samples using TRIzol Reagent	0	2008-12-12	2008-12-12
L2CJPT	Detection of African swine fever virus DNA using the isothermal ASFV vp72 655T Invader squared assay	0	2008-12-12	2008-12-12
L7EQ2N	BTV SEROTYPE 4 SYBR GREEN RT-PCR	0	2008-12-12	2008-12-12
L511QP	African Horse Sickness TaqMan RT-PCR	0	2008-12-12	2008-12-12
L4XNQ1	Boom-silica RNA extraction	0	2008-12-12	2008-12-12
L4X1BS	Preparation of Silica particles for nucleic acid extraction	0	2008-12-12	2008-12-12
L4VLBT	Slope/end point analysis of Invader data	0	2008-12-12	2008-12-12
L4TJ51	Real-time PCR detection and quantification of porcine viruses using molecular beacons	0	2008-12-12	2008-12-12
Q8MFS2	Protocol discussion	0	2009-01-07	2009-01-07
QAREVV	Protocol discussion	0	2009-01-08	2009-01-08
TLC5N8	Dr	0	2009-02-17	2009-02-17
TLC785	PLa	0	2009-02-17	2009-02-17
19J3P4Q	Collecting Plasma From Whole Blood	0	2009-08-31	2009-08-31
19J5LGB	Collection of Blood Serum	0	2009-08-31	2009-08-31
1BRK98A	Collection of white blood cells	0	2009-09-01	2009-09-01
1A04VLX	Processing of blood specimens	0	2009-09-02	2009-09-02
192208C	Processing of Solid Tissue Specimens	0	2009-09-03	2009-09-03
1A05N37	Processing of urine and buccal cells	0	2009-09-03	2009-09-03
1BXQD00	Collecting and Processing Saliva	0	2009-09-03	2009-09-03
1A0664L	Processing of cervical cells	0	2009-09-03	2009-09-03
1A06BCV	Processing of hair and nails	0	2009-09-03	2009-09-03
1DK6PVK	Radioimmunoprecipitation assay buffer (RIPA)	0	2009-09-23	2009-09-23
1FYH9CD	Rat and Mouse kidneys for LCM-proteomics	0	2009-10-22	2009-10-22
1GK1SHN	CyQUANT® NF	0	2009-10-29	2009-10-29
1GK6YRX	High salt buffer	0	2009-10-29	2009-10-29
1GK7EXB	Low salt buffer	0	2009-10-29	2009-10-29
1GKF4F4	Adhesion Assay Protocol	0	2009-10-29	2009-10-29
1GLJDF9	Immunofluorescence stain of cells in Permanox chambers	0	2009-11-01	2009-11-01
1GK7JEE	Immunoprecipitation	0	2009-11-01	2009-11-01
1GSVH8H	Immunofluorescence stain of cells on chamber slides	0	2009-11-01	2009-11-01
1GSX2AB	Hank´s buffer	0	2009-11-01	2009-11-01
1H1XRB8	Poly-L-lysine solution	0	2009-11-09	2009-11-09
1FYVTBM	Tissue preparation for Mass Spectrometry Imaging	0	2009-11-23	2009-11-23
1HFY4GV	Pre-analysis treatment for MALDI mass spectrometry imaging	0	2009-11-23	2009-11-23
1HPVN24	Preparation of ionic matrix for MALDI-Imaging of peptides	0	2009-11-23	2009-11-23
1HPXB0D	Preparation of ionic matrix for MALDI-Imaging of proteins	0	2009-11-23	2009-11-23
1KHEC7H	GMO specific real-time PCR system	0	2009-12-04	2009-12-04
1KNEEQ9	Quantitation of maize line MON 863	0	2009-12-06	2009-12-06
1KNFEP0	Quantitation of maize line MON 863	0	2009-12-06	2009-12-06
1KNFT6E	Quantitation of maize line MON 863	0	2009-12-06	2009-12-06
1KNG8BW	Real-time PCR for quantitative analysis of MON 863 maize	0	2009-12-06	2009-12-06
1KNHV0E	Buffers and solutions for quantitation of maize line MON 863	0	2009-12-06	2009-12-06
1KPG3VR	Real-time PCR Real-time PCR for quantitative analysis of T25 maize	0	2009-12-06	2009-12-06
1KPFSHQ	Procedures for quantitation of T25	0	2009-12-06	2009-12-06
1KPFHNL	Quantitation of maize line T25	0	2009-12-06	2009-12-06
1KPJMEL	Qquantitation of maize 59122	0	2009-12-07	2009-12-07
1KPNHTH	Procedures for quantitation of maize 59122	0	2009-12-07	2009-12-07
1KPN5K1	RT-PCR analysis of maize 59122	0	2009-12-07	2009-12-07
1KV3PBF	Quantitation of cotton 281-24-236	0	2009-12-08	2009-12-08
1KV4EC2	Procedures for quantitation of cotton 281-24-236	0	2009-12-08	2009-12-08
1KNQ0MP	Sampling and DNA extraction of maize TC15O7	0	2009-12-22	2009-12-22
1KNSRCL	Description of methods	0	2009-12-22	2009-12-22
1KNXC67	Experimental testing of the DNA extraction method	0	2010-03-13	2010-03-13
1QQ5NKF	Procedures for quantitation of cotton 3006-210-23	0	2010-03-13	2010-03-13
1RNMM1Q	Quantitation of cotton 3006-210-23	0	2010-03-13	2010-03-13
1S4SL9R	Procedures for quantification of Rice Line LLRICE62	0	2010-03-13	2010-03-13
1T9QCFT	Quantification of Rice Line LLRICE62	0	2010-03-13	2010-03-13
1T9TPLP	Procedures for quantitation of maize 59122	0	2010-03-13	2010-03-13
1KNQRED	Experimental Validation	0	2010-03-13	2010-03-13
1TP36XR	INVASION ASSAY BD Matrigel™	0	2010-03-13	2010-03-13
1TP6KJQ	Immunostaining of Cryostat Tissue sections	0	2010-03-13	2010-03-13
1TRMAN1	Purification of miRNA from flow-through	0	2010-03-14	2010-03-14
1V9BDC4	LDH Assay	0	2010-03-20	2010-03-20
1V9C2SB	Recipie for Lysis buffer	0	2010-03-20	2010-03-20
1V9FG4S	DNA isolation from bloodspots	0	2010-03-20	2010-03-20
1VB6T8R	MTT solution	0	2010-03-21	2010-03-21
1VB743D	Solubilization buffer	0	2010-03-21	2010-03-21
1VB7RPF	MTT assay	0	2010-03-21	2010-03-21
1VBE2CV	DNA extraction from parafin-embedded tissue	0	2010-03-21	2010-03-21
1VBFKEV	Isolation of DNA from laser‐microdissected tissue	0	2010-03-21	2010-03-21
1VBM98R	Immunohistochemistry Protocol	0	2010-03-21	2010-03-21
1VBVX4C	PI-solution	0	2010-03-21	2010-03-21
1VBVX85	FACS-protocol	0	2010-03-21	2010-03-21
1VBXNC6	Caspase reaction mix	0	2010-03-21	2010-03-21
1VC07B7	Caspase lysis buffer	0	2010-03-21	2010-03-21
1VC0WNJ	Effector caspase activation assay	0	2010-03-21	2010-03-21
1VEPSEX	PCR-protocol	0	2010-03-22	2010-03-22
1VGTJDL	Lysis buffer	0	2010-03-23	2010-03-23
1VGVN5H	Western Blot	0	2010-03-23	2010-03-23
1VGYAYS	Solutions and buffers for ISH	0	2010-03-23	2010-03-23
1VH5RCT	miRNA and mRNA in situ hybridization	0	2010-03-23	2010-03-23
1VERKAR	Calcium binding assay	0	2010-03-23	2010-03-23
1YY2MX3	NimbleGen targeted resequencing	0	2010-07-19	2010-07-19
1XQ81H8	Quantitation of maize 59122	0	2010-07-19	2010-07-19
2AEQQAD	Validation of the quantitation of maize line MON 810 DNA	0	2010-10-25	2010-10-25
27JEJMW	Procedures for quantitation of maize line MON 863	0	2010-10-25	2010-10-25
29P2FHN	Quantitation of maize line MON 863	0	2010-10-25	2010-10-25
2GTPKG9	Antibody Microarrays: Label plasma or serum samples	0	2010-12-17	2010-12-17
1T98QC3	PRINTING OF PROTEIN MICROARRAYS FROM DNA MICROARRAYS	0	2011-03-23	2011-03-23